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KMID : 1134820090380040502
Journal of the Korean Society of Food Science and Nutrition
2009 Volume.38 No. 4 p.502 ~ p.508
Antimicrobial Activity of Ethanol Extract from Sargassum thunbergii
Lee So-Young

Song Eu-Jin
Kim Koth-Bong-Woo-Ri
Yoon So-Young
Kim Seo-Jin
Lee So-Jeong
Hong Yong-Ki
Lim Sung-Mee
Ahn Dong-Hyun
Abstract
Antimicrobial activity of Sargassum thunbergii was determined by paper disc assay and minimum concentration inhibitor (MIC) test. A water extract of S. thunbergii did not show the antimicrobial activity, but an ethanol extract of S. thunbergii (SHE) inhibited Serratia liquefaciens, Salmonella Typhimurium, Pseudomonas aerogenosa and all of the tested gram-positive bacteria at 4 §·/§¢. Especially, Bacillus subtilis, Clostridium perfringens and Listeria monocytogenes were susceptible to SHE. As the results of MIC test, SHE inhibited the growth of B. subtilis, Staphylococcus aureus and Listeria monocytogenes at concentration of 0.1~0.3%, and inhibited C. perfringens at 0.01%. In the thermal and pH stability test for SHE, antibacterial activities of SHE were maintained when the SHE was treated at 121oC for 15 minutes or under pH 2~8. SHE was partitioned in the order of n-hexane, chloroform, ethyl acetate and butanol. As the results of the MIC test for each obtained fraction, no fraction exhibited higher antibacterial activity than that of the crude SHE. However, a mixture of chloroform, ethylacetate and ethanol fractions showed higher antibacterial activity than SHE.
KEYWORD
algae, Sargassum thunbergii, antibacterial activity
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